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Primary Antibodies
XBP1 Antibody
Background
XBP1 Antibody. This antibody is expected to recognize isoform XBP1(S) (NP_001073007.1) only.
Additional Names
XBP1, X-box binding protein 1, TREB5, XBP2, OTTHUMP00000028682, X-box-binding protein-1
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Description
Left: Western blot analysis of XBP1 in nuclear HeLa lysate (35μg protein in RIPA buffer) using XBP1 antibody (1μg/mL).
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Source
XBP1 antibody was raised against a synthetic peptide of XBP1.
Purification
XBP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
XBP1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
XBP1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-606P - XBP1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. XBL-10433 - HeLa Nuclear Lysate
Species Reactivity
H, M, D
Protein Accession Number
NP_001073007.1
Short Description
XBP1 / TREB5
This product belongs to the following categories:
Related Products
References- Yokouchi M, Hiramatsu N, Hayakawa K, Okamura M, Du S, Kasai A, Takano Y, Shitamura A, Shimada T, Yao J, Kitamura M. Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response. J Biol Chem. 2007 Dec 17; [Epub ahead of print]
Datasheet 08-01W
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