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Primary Antibodies
XBP-1 Monoclonal Antibody
Background
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Additional Names
XBP-1 (3H1G4), X box binding protein 1, Tax-responsive element binding protein 5
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Description
Left: Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/ml.
Below: Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/ml.
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Other Product Images
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Source
Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
Purification
Immunoaffinity chromotography purified IgG
Clonality / Clone
This is a monoclonal antibody. (Clone 3H1G4)
Host
XBP-1 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
Application
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
XBP-1 monoclonal antibody can be stored at 4˚C, stable for one year.
Positive Control
- Cat. No. 95-109 - XBP-1 Recombinant Protein
Species Reactivity
H, M, R
Short Description
X box binding protein 1
This product belongs to the following categories:
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References- Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
- Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
- Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
- Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
Datasheet 0801-W
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