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Primary Antibodies
XBP-1 Antibody
Background
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).
Additional Names
XBP-1 (CT), X box binding protein 1, Tax-responsive element binding protein 5
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Description
Left: Western blot analysis of XBP-1 in PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Below: Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/ml.
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Other Product Images
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Source
XBP-1 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human XBP-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
XBP-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human XBP-1 (C-Terminus) Peptide (Cat. No. 3685P)
Application
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3685P - XBP-1 Peptide
Storage
XBP-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
60416406
Protein Accession Number
P17861
Short Description
(CT) An ER signaling protein of the unfolded protein response pathway
This product belongs to the following categories:
Related Products
References- Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
- Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
- Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
- Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
Datasheet 08-02W
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