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Primary Antibodies
XAF-1 Antibody
Background
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).
Additional Names
XAF-1
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Description
Left: Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/ml, respectively.
Below: Immunohistochemical staining of human spleen tissue using XAF-1 antibody at 2 μg/ml.
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Other Product Images
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Source
XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
XAF-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human XAF-1 (C-Terminus) Peptide (Cat. No. 3207P)
Application
XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 μg/ml. A 32 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3207P - XAF-1 Peptide
Storage
XAF-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M
Protein GI Number
1869901
Protein Accession Number
CAA68030
Short Description
Inhibitor of XIAP
This product belongs to the following categories:
Related Products
References- Liston P, Fong W, Kelly NL, et al. Identification of XAF1 as an antagonist of XIAP anticaspase activity. Nature Cell Biol. 2001;3:128-33.
- Deveraux QL, Takahashi R, Savesan GS, and Reed JC. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997;388:300-4.
- Fong WG, Liston P, Rajcan-Separovic E, et al. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 2000;70:113-122.
- Leaman DW, Chawla-Sarkar M, Vyas K, et al. Identification of X-linked inhibitor of apoptosis associated factor-1 as an interferon-stimulated gene that augments TRAIL/Apo2L-induced apoptosis. J. Biol. Chem. 2002;277:28504-11.
Datasheet 08-02W
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