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Primary Antibodies
VPS53 Antibody
Background
The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
Additional Names
VPS53 (IN), Vacuolar protein sorting 53, HCCS1
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Description
Left: Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunofluorescence of LASS5 in human brain tissue with LASS5 antibody at 20 μg/ml.
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Source
VPS53 antibody was raised against a 18 amino acid peptide from near the center of human VPS53.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
VPS53 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IF
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4595P - VPS53 Peptide
Storage
VPS53 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
119611064
Protein Accession Number
EAW90658
Short Description
(IN) A vacuolar sorting protein
This product belongs to the following categories:
Related Products
References- Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol. 1991; 170:43-65.
- Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res. 2005; 306:22-34.
- Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
Datasheet 08-03W
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