|
Primary Antibodies
VGF Antibody
Background
VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.
Additional Names
VGF, Neurosecretory protein VGF, VGF nerve growth factor inducible
|
Description
Left: Western blot analysis of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of VGF in human brain with VGFantibody at 5 μg/ml.
|
|
Other Product Images
|
Source
VGF antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human VGF.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
VGF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
VGF antibody can be used for detection of VGF by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4611P - VGF Peptide
Storage
VGF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
17136078
Protein Accession Number
NP_003369
Short Description
Nerve growth factor inducible protein
This product belongs to the following categories:
Related Products
References- Levi A, Eldridge JD, and Paterson BM. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 1985; 229:393-5.
- Possenti R, Eldridge JD, Paterson BM, et al. A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulatory pathway. EMBO J. 1989; 8:2217-23.
- Hahm S, Mizuno TM, Wu TJ, et al. Targeted deletion of the Vgf gene indicates that the encoded secretory peptide precursor plays a novel role in the regulation of energy balance. Neuron 1999; 23:537-48.
- Thakker-Varia S, Krol JJ, Nettleton J, et al. The neuropeptide VGF produces antidepressant-like behavioral effects and enhances proliferation in the hippocampus. J. Neurosci. 2007; 27:12156-67.
Datasheet 08-03W
|
|
