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Primary Antibodies
UEV1A Antibody
Background
Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
Additional Names
UEV1A
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Description
Left: Western blot analysis of UEV1A in Jurkat cell lysates with UEV1A antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
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Source
UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
UEV1A antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus UEV1A Peptide (Cat. No. 3375P)
Application
UEV1A antibody can be used for detection of UEV1A by Western blot at 1 to 2 μg/ml. Anti-UEV1A may also recognize other isoforms of UEV1A.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3375P - UEV1A Peptide
Storage
UEV1A antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
40806167
Protein Accession Number
NP_954595
Short Description
a ubiquitin conjugating enzyme
This product belongs to the following categories:
Related Products
References- Sancho E, Vila MR, Sanchez-Pulido L, et al. Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells. Mol. Cell Biol. 1998; 18:576-89.
- Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Deng L, Wang C, Spencer E, et al. Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61.
Datasheet 08-02W
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