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Primary Antibodies
UBC13 Antibody
Background
Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
Additional Names
UBC13
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Description
Left: Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 μg/ml.
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Other Product Images
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Source
UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
UBC13 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus UBC13 / Ubiquitin-conjugating enzyme E2N Peptide (Cat. No. 3373P)
Application
UBC13 antibody can be used for detection of UBC13 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3373P - UBC13 Peptide
Storage
UBC13 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
- Cat. No. 1409 - Mouse Thymus Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
30582585
Protein Accession Number
AAP35519
Short Description
Ubiquitin-conjugating enzyme 13
This product belongs to the following categories:
Related Products
References- Brusky J, Zhu Y, and Xiao W. UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr. Genet. 2000; 37:168-74.
- Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Deng L, Wang C, Spencer E, et al. Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61.
Datasheet 08-02W
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