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Primary Antibodies
Tyrosine Hydroxylase, Sheep Antibody
Background
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.
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Description
Left: Tyrosine Hydroxylase, Sheep Antibody
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Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
Tyrosine Hydroxylase antibody was raised in sheep.
Please use anti-sheep secondary antibodies.
Immunogen
Sheep anti-TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma. Immune sera from several bleeds were pooled and an IgG fraction was isolated by caprylic acid and ammonium sulfate fractionation. The antibody was further purified by affinity chromatography on a tyrosine hydroxylase-AminoLink Plus column. The antibody is predominantly of the IgG1 subclass.
Application
Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB). The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
Tested Application
WB, IHC, IF
Buffer
Tyrosine Hydroxylase antibody is supplied as affinity purified polyclonal antibody, 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. There is adequate amount of material to conduct 10-mini Western Blots.
Storage
For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Species Reactivity
H, M, R, D, B
Short Description
Tyrosine Hydroxylase Pan-Specific
This product belongs to the following categories:
Related Products
References- Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., “Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies,” Neuroscience, 82 (1998) 727 – 738.
- Haycock, J.W. “Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies,” J. Neurochem. 60 (1993) 493 – 502.
- Haycock, J.W., “Four forms of tyrosine hydroxylase are present in human adrenal medulla,” J. Neurochem. 56 (1991) 2139 - 2142.
- Haycock, J.W., “Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody,” Anal. Biochem. 181 (1989) 259 - 266.
Datasheet 0802-W
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