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Primary Antibodies
Tyrosine Hydroxylase (Phospho-Ser19) Antibody
Background
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.
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Description
Left: Tyrosine Hydroxylase (Phospho-Ser19) Antibody
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Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
Tyrosine Hydroxylase antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Rabbit anti-TH (Ser19) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser19 of rat tyrosine hydroxylase (TH). Rabbit anti-TH (Ser19) was purified by sequential chromatography on phospho-and non-phosphopeptide affinity columns.
Application
Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser19 in PC-12 cells. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.
Tested Application
WB, IHC, IF
Buffer
Tyrosine Hydroxylase antibody is supplied as affinity purified polyclonal antibody, 100 μl in 150 mM NaCl, 10 mM HEPES, 100 μg per ml BSA and 50% glycerol, pH 7.5., and there is adequate amount of material to conduct 10-mini Western Blots.
Storage
For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Species Reactivity
R
This product belongs to the following categories:
Related Products
References- Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., “Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina,” J. Chem. Neuroanat., 19 (2000) 105 - 116.
- Haycock, J. W., Lew, J. Y., Garcia-Espana, A., Lee, K. Y., Harada, K., Meller, E., and Goldstein, M., “Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis,” J. Neurochem., 71 (1998) 1670 – 1675.
- Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., “Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies,” Neuroscience, 82 (1998) 727 – 738.
Datasheet 0802-W
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