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XA-1017


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GeneDireX Molecular Weight Marker


Antibody Production


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Primary Antibodies

Troy (MAb Giu.Li) Antibody

Background

*Please allow 3-5 days for delivery time.*

Additional Names

taj, tnfrsf

Source

Troy (monoclonal Giu.Li) antibody was raised against recombinant human Troy;Fc (aa. 1-168).

Purification

Troy (monoclonal Giu.Li) antibody was purified to ≥95% in SDS-PAGE and is supplied at a concentration of 0.3 mg/1ml. 

Clonality / Clone

This is a monoclonal antibody.

Isotype

Rat IgG2a

Host

Troy antibody was raised in rat.

Please use anti-rat IgG secondary antibodies

Immunogen

The immunogen is recombinant human Troy;Fc, amino acids 1-168.  The cysteine-rich domain of human Troy (TAJ) (aa. 1-168) is fused to the Fc portion of human IgG1.

Application

Troy antibody can be used to recognize human Troy by flow cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of monoclonal antibody to Troy (Giu.Li). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.  

Tested Application

FC

Buffer

Troy antibody is supplied as a liquid in PBS containing 0.02% sodium azide.

Storage

Troy antibody can be stored at 4˚C, stable for one year.

Species Reactivity

H

Short Description

monoclonal to Troy (Giu.Li)

This product belongs to the following categories:

References

  1. TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles: T. Kojima, et al.; J. Biol. Chem. 275, 20742 (2000).
  2. TAJ, a novel member of the tumor necrosis factor receptor family, activates the c-Jun N-terminal kinase pathway and mediates caspase-independent cell death: M.T. Eby, et al.; J. Biol. Chem. 275, 15336 (2000).
  3. TRAF6-deficient mice display hypohidrotic ectodermal dysplasia: A. Naito, et al.; PNAS 99, 8766 (2002).


Datasheet 0802-W

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