|
Primary Antibodies
TWEAK Antibody
Background
TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane (1,2). TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis (3), induction of inflammatory cytokines (4), in addition to stimulation of apoptosis (5). The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains (1). Despite its predicted molecular weight of 27kDa, TWEAK may migrate at aberrant positions in SDS-PAGE.
Additional Names
TWEAK, TNF-related weak inducerof apoptosis, TNF ligand superfamily member 12
|
Description
Left: Western blot analysis of (A) 5 ng, (B) 25 ng and (C) 50 ng of recombinant TWEAK with TWEAK antibody at 1 μg/ml.
Below: Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
TWEAK antibody was raised against recombinant human TWEAK.
Purification
Purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
TWEAK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Recombinant human TWEAK (Genbank accession No. NP_003800)
Application
TWEAK antibody can be used for the detection of TWEAK by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3791P - TWEAK Peptide
Storage
TWEAK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4507597
Protein Accession Number
NP_003800
Short Description
A TNF-a related protein
This product belongs to the following categories:
Related Products
References- Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
- Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem. 1997; 272:32401-10.
- Lynch CN, Wang YC, Lund JK, et al. TWEAK induces angiogenesis and proliferation of endothelial cells. J. Biol. Chem. 1999; 274:8455-9.
- Chicheportiche Y, Chicheportiche R, Sizing I, et al. Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies. Arthr. Res. 2002; 4:126-33. 5. Kaplan MJ, Lewis EE, Shelden EA, et al. The apoptotic ligands TRAIL, TWEAK, and Fas ligand mediate monocyte death induced by autologous lupus T cells. J. Immunol. 2002; 169:6020-9.
Datasheet 08-02W
|
|
