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Primary Antibodies
TWA1 Antibody
Background
TWA1 was identified through a two hybrid-associated protein screen with RanBPM. TWA1 is well conserved through evolution and is localized within the nucleus. It interacts with RanBP9 and comprises a protein complex with RanBPM and Muskelin. TWA1 was found to possess the LisH-CTLH motif which is detected in proteins involved in microtubule dynamics, cell migration, nucleokinesis and chromosome segregation. These functions overlap with functions suggested for the RanGTPase cycle. Recent findings suggested that there is an as yet uncovered function of the RanGTPase cycle.
Additional Names
TWA1,Two hybrid-associated protein 1 with RanBPM
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Description
Left: Western blot analysis of TWA1 in human brain tissue lysate with TWA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Source
TWA1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human TWA1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TWA1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TWA1 antibody can be used for detection of TWA1 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5305P - TWA1 Peptide
Storage
TWA1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
8923557
Protein Accession Number
NP_060366
Short Description
Two hybrid-associated protein 1 with RanBPM
This product belongs to the following categories:
References- Umeda M, Nishitani H, and Nishimoto T. A novel nuclear protein, Twa1, and Muskelin comprise a complex with RanBPM. Gene 2003; 303:47-54.
- Kobayashi N, Yang J, Ueda A, et al. RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex. Gene 2007; 396:236-47.
Datasheet 09-01W
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