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Primary Antibodies
TTC5 Antibody
Background
Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats (1). Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 (2) and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5 (3), suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.
Additional Names
TTC5, TTC5, Tetratricopeptide repeat protein 5, TPR repeat protein 5
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Description
Left: Western blot analysis of TTC5 in RAW264.7 cell lysate with TTC5 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of TTC5 in mouse brain tissue with TTC5 antibody at 10 μg/ml.
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Other Product Images
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Source
TTC5 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human TTC5.
Purification
Immunoaffinity purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
TTC5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TTC5 Peptide (Cat. No. 3053P)
Application
TTC5 antibody can be used for the detection of TTC5 by Western blot at 0.5 – 1 μg/ml. (Optimal dilution should be determined by user).
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3053P - TTC5 Peptide
Storage
TTC5 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1283 - RAW264.7 Cell Lysate
Species Reactivity
H, M
Protein GI Number
67462006
Protein Accession Number
Q8N0Z6
Short Description
A tetratricopeptide repeat containing protein 5
This product belongs to the following categories:
Related Products
References- Blatch GL and Lassle M. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. BioEssays 1999; 21:932-9
- Song Y and Masison DC. Independent regulation of Hsp70 and Hsp90 chaperones by Hsp70/Hsp90-organizing protein Sti1 (Hop1). J. Biol. Chem. 2005; 280:34178-85.
- Yang J, Roe SM, Cliff MJ, et al. Molecular basis for TPR domain-mediated regulation of protein phosphatase 5. EMBO J. 2005; 24:1-10.
Datasheet 08-02W
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