|
Primary Antibodies
TRBP1 Antibody
Background
The human trans-activation response (TAR) RNA binding protein 1 (TRBP1) was initially identified as a protein that binds to the HIV-1 TAR RNA and activates the long terminal repeat (LTR) expression in the absence and presence of the viral trans-activator Tat. This binding is enhanced by the presence of co-factors such as elongation factor 1α (EF-1α), polypyrimidine tract-binding protein (PTB), and the chaperonin-like protein stimulator of TAR RNA-binding proteins (SRB). TRBP1 may act to disengage RNA polymerase II from TAR during transcriptional elongation if the RNA polymerase stalls at the TAR during transcriptional elongation.
Additional Names
TRBP1 (IN), TAR RNA binding protein 1, TARBP1, TRP-185, TRP-1
|
Description
Left: Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 μg/ml.
|
Source
TRBP1 antibody was raised against a 17 amino acid peptide from near the center of human TRBP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TRBP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TRBP1 antibody can be used for detection of TRBP1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4011P - TRBP1 Peptide
Storage
TRBP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1212 - 3T3 Cell Lysate
Species Reactivity
H, M
Protein GI Number
110825988
Protein Accession Number
NP_005637
Short Description
(IN) TAR RNA binding protein 1
This product belongs to the following categories:
Related Products
References- Gatignol A, Buckler-White A, Berkhout B, et al. Characterization of a human TAR REN-binding protein that activates the HIV-1 LTR. Science 1991; 251:1597-1600.
- Wu-Baer F, Lane WS, and Gaynor RB. Identification of a group of cellular cofactors that stimulate the binding of RNA polymerase II and TRP-185 to human immunodeficiency virus 1 TAR RNA. J. Biol. Chem. 1996; 271:4201-8.
- Wu-Baer F, Lane WS, and Gaynor RB. The cellular factor TRP-185 regulates RNA polymerase II binding to HIV-1 TAR RNA. EMBO J. 1995; 14:5995-6009.
(07-01D)
Datasheet 08-02W
|
|
