|
Detection Sets
TRAIL and Receptor Detection Set
Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organism. It is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family which is expressed in a variety of human tissues. Similar to TNF and Fas ligand, TRAIL induces apoptosis and NF-κB activation in many tissues and cells. Its receptors include the death domain containing proteins DR4 and DR5, both of which mediate TRAIL induced cell death. Two decoy receptors, DcR1 and DcR2, have also been identified. Both of these proteins contain an extracellular TRAIL binding domain but lack intracellular signaling domains. Overexpression of either DcR1 or DcR2 attenuates TRAIL-induced apoptosis.
|
Description
For images please see PDF data sheet
|
Kit Contents
TRAIL Antibody, Cat. No. 1113 (50μg)
DR4 Antibody, Cat. No. 1139 (50μg)
DR5 Antibody, Cat. No. 2019 (50μg)
DcR1 Antibody, Cat. No. 2179 (50μg)
DcR2 Antibody, Cat. No. 2021 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of TRAIL and its receptors by Western blot at 2 μg/ml to 1 μg/ml and Immunohistochemistry. Whole cell lysate from HeLa cells can be used as a positive control for each of these antibodies. For research use only.
Tested Application
WB, IHC
Blocking Peptide
TRAIL Peptide, Cat. No. 1113P
DR4 Peptide, Cat. No. 1139P
DR5 Peptide, Cat. No. 2019P
DcR1 Peptide, Cat. No. 2179P
DcR2 Peptide, Cat. No. 2021P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -4˚C.
Positive Control
- HeLa Lysate, Cat. No. 1201
Species Reactivity
see datasheet
Short Description
TRAIL, DR4, DR5, DcR1, and DcR2
This product belongs to the following categories:
Related Products
References- Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995; 3:673-82.
- Pitti RM, Marsters SA, Ruppert S, et al. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996; 271:12687-90.
- Schneider P, Thome M, Burns K, et al. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-κB. Immunity 1997; 7:831-6.
- Pan G, O’Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL. Science 1997; 276:111-3.
Datasheet 0803-W
|
|
