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Primary Antibodies
TRAF6 Antibody
Background
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-?B (1,2). TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families (3). TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands (4). It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) (5) or by other TLR adaptor molecules such as TRIF (6). Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK (7).
Additional Names
TRAF6 (CT), TRAF6
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Description
Left: Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/ml in the presence (A) or absence (B) of 1 μg blocking peptide.
Below: Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/ml.
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Other Product Images
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Source
TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
TRAF6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TRAF6 (C-Terminus) Peptide (Cat. No. 3129P)
Application
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 to 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3129P - TRAF6 Peptide
Storage
TRAF6 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
4759254
Protein Accession Number
NP_004611
Short Description
(CT) TNF receptor associated factor
This product belongs to the following categories:
Related Products
References- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.
- Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.
- Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63.
Datasheet 08-02W
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