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Primary Antibodies
TRAF3 Antibody
Background
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1 (2,3). Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB (4). Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-β receptor (LTβR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTβR.
Additional Names
TRAF3 (NT), TNF receptor-associated factor 3 / CD40 binding protein / LMP1 associated protein 1 / CD40BP / LAP1
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Description
Left: Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/ml.
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Other Product Images
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Source
TRAF3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TRAF3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TRAF3 (N-Terminus) Peptide (Cat. No. 3601P)
Application
TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3601P - TRAF3 Peptide
Storage
TRAF3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
22027618
Protein Accession Number
NP_663777
Short Description
(NT) TNF receptor associated factor-3
This product belongs to the following categories:
Related Products
References- Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) – a family of adaptor proteins that regulate life and death.Genes Dev. 1998; 12:2821-30.
- Cheng G, Cleary AM, Ye Z, et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 1995; 267:1494-8.
- Mosialos G, Birkenbach M, Yalamanchili R, et al. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995; 80:389-99.
- van Eyndhoven WG, Gamper CJ, Cho E, et al. TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0κB activation. Mol. Immunol. 1999; 36:647-58.
Datasheet 08-02W
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