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Primary Antibodies
TRAF2 Antibody
Background
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress (1). TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved. For example, TRAF2 and TRAF3 directly bind to the CD40, a TNF receptor superfamily member involved in inducing B cell immunity (2), and are critical for NF-κB activation in mouse B lymphocytes (3). TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells (4). TRAF2 also interacts with the TRFR superfamily member lymphotoxin-b receptor (LTbR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac (5).
Additional Names
TRAF2 (CT), TNF receptor-associated factor 2
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Description
Left: Western blot analysis of TRAF2 in mouse liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of TRAF2 in mouse liver tissue with TRAF2 antibody at 10 μg/ml.
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Other Product Images
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Source
TRAF2 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human TRAF2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TRAF2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TRAF2 (C-Terminus) Peptide (Cat. No. 3661P)
Application
TRAF2 antibody can be used for the detection of TRAF2 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3661P - TRAF2 Peptide
Storage
TRAF2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
55959980
Protein Accession Number
CAI15106
Short Description
(CT) TNF receptor-associated factor 2
This product belongs to the following categories:
Related Products
References- Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) – a family of adaptor proteins that regulate life and death. Genes Dev. 1998; 12:2821-30.
- van Kooten C and Bancherau J. CD40-CD40 ligand. J. Leukoc. Biol. 2000; 67:2-17.
- Grech AP, Amesbury M, Chan T, et al. TRAF2 differentially regulates the canonical and noncanonical pathways of NF-kappaB activation in mature B cells. Immunity 2004; 21:629-42.
- Davies CC, Mak TW, Young LS, et al. TRAF6 is required for TRAF2-dependent CD40 signal transduction in nonhemopoietic cells. Mol. Cell. Biol. 2005; 25:9806-19.
Datasheet 08-02W
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