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Primary Antibodies
TOR Antibody
Background
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin (2,3). Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 4). TOR is normally associated with the regulatory proteins RAPTOR and GβL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis (4).
Additional Names
TOR, mTOR, FRAP, RAFT, RAPT
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Description
Left: Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/ml.
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Other Product Images
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Source
TOR antibody was raised against a 15 amino acid peptide from near the amino terminus of human TOR.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TOR antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus TOR / mTOR / FRAP / RAFT / RAPT Peptide (Cat. No. 3485P)
Application
TOR antibody can be used for the detection of TOR by Western blot at 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3485P - TOR Peptide
Storage
TOR antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1284 - L1210 Cell Lysate
Species Reactivity
H, M
Protein GI Number
4826730
Protein Accession Number
NP_004949
Short Description
Target of Rapamycin
This product belongs to the following categories:
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References- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
- Sabatini DM, Erdjument-Bromage H, Lui M, et al. RAFT1: a mammalian protein that binds to FKP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 1994; 78:35-43.
- Cardenas ME, Zhu D, and Heitman J. Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin. Curr. Opin. Nephrol. Hypertens. 1995; 4:472-7.
- Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
Datasheet 08-02W
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