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Primary Antibodies
TOCA-1 Antibody
Background
Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Additional Names
TOCA-1 (NT), Transducer of cdc42-dependent actin assembly 1, formin binding protein 1-like, FNBP1L
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Description
Left: Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 μg/ml.
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Source
TOCA-1 antibody was raised against a 15 amino acid peptide near the amino terminus of the human TOCA-1.
Clonality / Clone
This is a polyclonal antibody.
Host
TOCA-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 – 2 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
TOCA-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H,M,R
Protein Accession Number
NP_001020119
Data Sheet Version
08-02W
Related Products
References
- Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell 2004; 118:203-16.
- Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem. 2006; 281:29042-43.
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