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Primary Antibodies
TMEM16A Antibody
Background
Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC’s include the transmembrane proteins TMEM16A and TMEM16B. TMEM16A is expressed in epithelial cells of the kidney and lung, pancreas, and sensory neurons and its mRNA is seen in foregut, airway epithelia, and tracheal smooth muscle; mice lacking TMEM16A fail to survive past ten days and show aerophagia and little weight gain. TMEM16A is also overexpressed or amplified in multiple cancers associated with poor survival such as oral cancers and gastrointestinal stromal tumors, suggesting the development of CaCC modulators may be a viable therapeutic strategy.
Additional Names
TMEM16A, Anoctamin 1, calcium activated chloride channel, ano1, DOG1, TAOS1, ORAOV2
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Description
Left: Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/ml.
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Source
TMEM16A antibody was raised against a 17 amino acid peptide from near the amino terminus of human TMEM16A.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TMEM16A antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TMEM16A antibody can be used for detection of TMEM16A by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5419P - TMEM16A Peptide
Storage
TMEM16A antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1203 - A549 Cell Lysate
Species Reactivity
H, M
Protein GI Number
194306539
Protein Accession Number
NP_060513
Short Description
Anoctamin 1, calcium activated chloride channel
This product belongs to the following categories:
References- Schroeder BC, Cheng T, Jan YN, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell 2008; 134:1019-29.
- Rock JR, Futtner CR, and Harfe BD. The transmembrane protein TMEM16A is required for normal development of the murine trachea. Dev. Biol. 2008; 214:399-411.
- Huang X, Godfrey TE, Gooding WE, et al. Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma. Genes Chromosomes Cancer 2006; 45:10458-69.
- West RB, Corless CL, Chen X, et al. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGRA mutation status. Am. J. Pathol. 2004; 165:107-13.
Datasheet 09-01W
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