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Primary Antibodies
TLR9 Antibody
Background
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences (3) and are localized in intracellular acidic compartments such as the phagolysosome (4). Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection (5), TLR9 is strictly dependent on MyD88 (3).
Additional Names
TLR9 (IN), Toll-like receptor 9, CD289
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Description
Left: Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunocytochemistry of TLR9 in Jurkat cells with TLR9antibody at 2 μg/ml.
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Other Product Images
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Source
TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TLR9 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TLR9 (Intermediate Domain) Peptide (Cat. No. 3739P)
Application
TLR9 antibody can be used for detection of TLR9 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3739P - TLR9 Peptide
Storage
TLR9 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
21595773
Protein Accession Number
AAH32713
Short Description
(IN) Toll-like receptor
This product belongs to the following categories:
Related Products
References- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- Wagner H. The immunobiology of the TLR9 subfamily. Trends Immunol. 2004; 381-6
- Nishiya T and DeFranco AL. Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors. J. Biol. Chem. 2004; 279:19008-17.
Datasheet 08-02W
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