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Catalog Number:
49-767

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GeneDireX Molecular Weight Marker

Antibody Production

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Primary Antibodies

TLR9 Antibody

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (4) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TRL9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen.

Additional Names

TLR9, CD289, scri2a, tlr9, toll-like receptor 9, toll-like receptors 9

TLR9 Antibody
Description
Left: Immunohistochemistry staining of TLR9 in kidney (formalin-fixed paraffin-embedded) using TLR9 antibody.

Below: Western Blot analysis of TLR9 incell lysates from mouse intestine using TLR9 antibody (0.5μ/ml).

Other Product Images

Purification

Protein G Column

Clonality / Clone

This is a polyclonal antibody.

Isotype

IgG

Host

TLR9 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

TLR9 antibody was raised against amino acids 200-300 of TLR9. (Mouse)

Application

TLR9 antibody can be used for detection of TLR9 by WB (minimum working concentration: 5 μg/ml maximum working concentration: 1 μg/ml), FACS, IHC-parafin (minimum working concentration: 10 μg/ml).