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Primary Antibodies
TLR8 Monoclonal Antibody
Background
Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa (4,5). Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Like TLR7, TLR8 is localized to endosomal or lysosomal compartments and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA. Human but not murine TLR8 confers responsiveness to the antiviral compound R-848. The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
Additional Names
TLR8, MGC119599, MGC119600, tlr8, toll-like receptor 8, toll-like receptors 8
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Description
Left: Immunohistochemistry staining of TLR8 in brain, cortexTLR8 Monoclonal antibody.
Below: Western Blot analysis of TLR8 incell lysates from 293 transfected with human TLR8 using TLR8 monoclonal antibody (2μ/ml).
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Other Product Images
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Purification
Protein G Column
Clonality / Clone
This is a monoclonal antibody. Clone 44C143.
Isotype
IgG,k
Host
TLR8 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
TLR8 monoclonal antibody was raised against amino acids 750-850 of TLR8. (Human)
Application
TLR8 monoclonal antibody can be used for detection of TLR8 by WB (minimum working concentration: 1 μg/ml maximum working concentration: 3 μg/ml), FACS, IHC-parafin (maximum working concentration: 5 μg/ml).
Tested Application
WB, IHC, FACS
Buffer
TLR8 monoclonal antibody is supplied in PBS, 0.05% BSA, 0.005% sodium azide.
Storage
TLR8 monoclonal antibody can be stored at 4˚C or -20˚C. Avoid repeated freezing and thawing.
Species Reactivity
M
Formulation
Weight: 50 μg
Concentration: 0.5 mg/ml
Short Description
Toll-like Receptor 8
This product belongs to the following categories:
Datasheet 09-01W
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