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Primary Antibodies
TLR8 Antibody
Background
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (4,5). Like TLR7, TLR8 is localized to endosomal or lysosomal compartments (6) and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA (7). Human but not murine TLR8 confers responsiveness to the antiviral compound R-848 (8).
Additional Names
TLR8 (IN), TLR8, CD288
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Description
Left: Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of TLR8 in Daudi cells with TLR8 antibody at 2 μg/ml.
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Other Product Images
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Source
TLR8 antibody was raised against a peptide corresponding to 16 amino acids near the middle of human TLR8.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TLR8 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TLR8 (Intermediate Domain) Peptide (Cat. No. 3281P)
Application
TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3281P - TLR8 Peptide
Storage
TLR8 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M
Protein GI Number
20302168
Protein Accession Number
NP_619542
Short Description
(IN) Toll-like receptor protein
This product belongs to the following categories:
Related Products
References- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Datasheet 08-02W
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