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Primary Antibodies
TLR7 Antibody
Background
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (4,5). TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome (6) and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus (7). Activation of TLR7 by VSV results in stimulation of the immune response including IFNΑ secretion, suggesting the importance of TLR7 in virus recognition.
Additional Names
TLR7 (IN), TLR7
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Description
Left: Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 μg/ml.
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Other Product Images
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Source
TLR7 antibody was raised against a peptide corresponding to 14 amino acids near the middle of mouse TLR7.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
TLR7 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TLR7 (Intermediate Domain) Peptide (Cat. No. 3269P)
Application
TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3269P - TLR7 Peptide
Storage
TLR7 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M
Protein GI Number
18875360
Protein Accession Number
NP_573474
Short Description
(IN) Toll-like receptor protein
This product belongs to the following categories:
Related Products
References- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Datasheet 08-02W
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