|
Primary Antibodies
TLR7 Antibody
Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFNa secretion, suggesting the importance of TLR7 in virus recognition.
Additional Names
TLR7, tlr7, toll-like receptor 7, toll-like receptors 7
|
Description
Left: Immunohistochemistry staining of TLR7 in small intestine using TLR7 antibody.
Below: Western Blot analysis of TLR7 inRaw cell lysate using TLR7 antibody (1μ/ml).
|
|
Other Product Images
|
Purification
Protein G Column
Clonality / Clone
This is a polyclonal antibody.
Isotype
IgG
Host
TLR7 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
TLR7 antibody was raised against amino acids 706-728 of TLR7. (Human)
Application
TLR7 antibody can be used for detection of TLR7 by WB (minimum working concentration: 1 μg/ml maximum working concentration: 3 μg/ml), FACS, IHC-parafin (minimum working concentration: 5 μg/ml maximum working concentration: 5 μg/ml), IHC-frozen.
Tested Application
WB, IHC, FACS
Buffer
TLR7 antibody is supplied in PBS, 0.05% BSA, 0.05% sodium azide.
Storage
TLR7 antibody can be stored at 4˚C or -20˚C. Avoid repeated freezing and thawing.
Species Reactivity
M
Formulation
Weight: 50 μg
Concentration: 0.5 mg/ml
Short Description
Toll-like Receptor 7
This product belongs to the following categories:
Datasheet 09-01W
|
|
