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Primary Antibodies
TLR4 Antibody
Background
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) α/β and NF-κB (4-6). Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis (7).
Additional Names
TLR4 (NT), TLR4
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Description
Left: Western blot analysis of TLR4 in PC-3 cell lysates with TLR4 antibody at (A) 2.5 and (B) 5 μg/ml.
Below: Immunocytochemistry of TLR4 in K562 cells with TLR4 antibody at 2 μg/ml.
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Other Product Images
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Source
TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
TLR4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TLR4 (N-Terminus) Peptide (Cat. No. 3141P)
Application
TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 5 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3141P - TLR4 Peptide
Storage
TLR4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
19924149
Protein Accession Number
NP_612564
Short Description
(NT) Toll-like receptor
This product belongs to the following categories:
Related Products
References- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Datasheet 08-02W
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