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Detection Sets
TLR Detection Set
Background
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.
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Description
For images please see PDF data sheet
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Kit Contents
TLR1 Antibody (NT), Cat. No. 3639 (50μg)
TLR2 Antibody (NT), Cat. No. 3135 (50μg)
TLR3 Antibody (CT), Cat. No. 3643 (50μg)
TLR4 Antibody (NT), Cat. No. 3141 (50μg)
TLR5 Antibody (CT), Cat. No. 3649 (50μg)
TLR6 Antibody (IN), Cat. No. 3653 (50μg)
TLR7 Antibody (IN), Cat. No. 3269 (50μg)
TLR8 Antibody (IN), Cat. No. 3281 (50μg)
TLR9 Antibody (IN), Cat. No. 3739 (50μg)
TLR10 Antibody (IN), Cat. No. 3275 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of TLR adaptor proteins by Western blot at 1 μg/ml to 4 μg/ml and Immunohistochemistry/Immunocytochemistry. Positive control whole cell lysates are available for each of these antibodies. For research use only.
Tested Application
WB, IHC, ICC
Blocking Peptide
TLR1 Peptide, Cat. No. 3639P
TLR2 Peptide, Cat. No. 3135P
TLR3 Peptide, Cat. No. 3643P
TLR4 Peptide, Cat. No. 3141P
TLR5 Peptide, Cat. No. 3649P
TLR6 Peptide, Cat. No. 3653P
TLR7 Peptide, Cat. No. 3269P
TLR8 Peptide, Cat. No. 3281P
TLR9 Peptide, Cat. No. 3739P
TLR10 Peptide, Cat. No. 3275P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -4˚C.
Positive Control
- A-20 Lysate (for TLR2), Cat. No. 1288
Daudi Lysate (for TLR3, TLR7 and TLR8), Cat. No. 1224
Human Lymph Node Lysate (for TLR10), Cat. No. 1369
Jurkat Lysate (for TLR6 and TLR9), Cat. No. 1205
Mouse Spleen Lysate (for TLR1), Cat. No. 1406
PC-3 Lysate (for TLR4), Cat. No. 1216
THP-1 Lysate (for TLR5), Cat. No. 1208
Species Reactivity
see datasheet
Short Description
TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, and TLR10
This product belongs to the following categories:
Related Products
References- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol. Imm. 2004; 41:577-82.
Datasheet 0803-W
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