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Primary Antibodies
TIRAP Antibody
Background
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4,5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-κB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6,7)
Additional Names
TIRAP (IN), MAL
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Description
Left: Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody at 4 μg/ml.
Below: Immunohistochemistry of TIRAP in human heart tissue with TIRAP antibody at 20 μg/ml.
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Other Product Images
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Source
TIRAP antibody was raised against a peptide corresponding to amino acids near the middle of human TIRAP.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
TIRAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus TIRAP / MAL (Intermediate Domain) Peptide (Cat. No. 3155P)
Application
TIRAP antibody can be used for detection of TIRAP by Western blot at 4 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3155P - TIRAP Peptide
Storage
TIRAP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1219 - MCF7 Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H
Protein GI Number
15705407
Protein Accession Number
AAL05627
Short Description
(IN) Toll-like receptor adaptor protein
This product belongs to the following categories:
Related Products
References- Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Datasheet 08-02W
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