|
Primary Antibodies
TIP47 Antibody
Background
Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
Additional Names
TIP47 (CT), Tail-interacting protein 47, mannose-6-phosphate receptor binding protein 1, placental protein 17
|
Description
Left: Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
TIP47 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human TIP47.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TIP47 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3881P - TIP47 Peptide
Storage
TIP47 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
- Cat. No. 1464 - Rat Liver Tissue Lysate
Species Reactivity
H, R
Protein GI Number
68846601
Protein Accession Number
O60664
Short Description
(CT) golgi, intracellular traffic, rab9
This product belongs to the following categories:
Related Products
References- Barbero P, Bittova L, and Pfeffer SR. Visualization of Rab9-mediated vesicle transport from endosomes to the trans Golgi in living cells. J. Cell Biol. 2002; 156:511-8.
- Carroll KS, Hanna J, Simon I, et al. Role of the Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 2001; 292:1373-7.
- Aivazian D, Serrano RL, and Pfeffer S. TIP47 is a key effector for Rab9 localization. J. Cell Biol. 2006; 173:917-26.
- Ganley IG, Carroll K, Bittova L, et al. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol. Biol. Cell 2004; 15:5420-30.
Datasheet 08-02W
|
|
