|
Primary Antibodies
TIM-4 Antibody
Background
The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.
Additional Names
TIM-4 (IN), T cell immunoglobulin and mucin domain containing protein 4, T cell membrane protein 4, SMUCKLER
|
Description
Left: Western blot analysis of TIM-4 in Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
TIM-4 antibody was raised against a 16 amino acid peptide from near the center of human TIM-4.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TIM-4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3815P - TIM-4 Peptide
Storage
TIM-4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M
Protein GI Number
226529863
Protein Accession Number
NP_612388
Short Description
(IN) T cell immunoglobulin and mucin domain containing protein 4
This product belongs to the following categories:
Related Products
References- Meyers JH, Sabatos CA, Chakravarti S, et al. The TIM family regulates autoimmune and allergic diseases. Trends Mol. Med. 2005; 11:362-9.
- Meyers JH, Chakravarti S, Schlesinger D, et al. TIM-4 is the ligand for TIM-1, and the TIM-1-TIM4 interaction regulates T cell proliferation. Nat. Immunol. 2005; 6:455-64.
- Kuchroo VK, Umetsu DT, DeKruyff RH, et al. The TIM gene family: emerging roles in immunity and disease. Nat. Rev. Immunol. 2003; 3:454-62.
- Shakhov AN, Rybtsov S, Tumanov AV, et al. SMUCKLER/TIM4 is a distinct member of TIM family expressed by stromal cells of secondary lymphoid tissues and associated with lymphotoxin signaling.
Datasheet 08-02W
|
|
