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Primary Antibodies
TIM-3 Antibody
Background
TIM-3 Antibody.
Additional Names
HAVCR2, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIM3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3
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Description
Left: Western blot analysis of TIM-3 in human tonsil lysate (35μg protein in RIPA buffer) using TIM-3 antibody (0.3μg/mL).
Below: HEK293 overexpressing TIM-3 and probed with TIM-3 antibody (mock transfection in first lane).
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Other Product Images
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Source
TIM-3 antibody was raised against a synthetic peptide of TIM-3.
Purification
TIM-3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
TIM-3 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 35kDa band observed in Human Tonsil, Thymus, Lymph Node and Spleen lysates (calculated MW of 33.4kDa according to NP_116171.3). In transfected HEK293 transiently expressing HAVCR2 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0μg/ml.
Tested Application
E, WB
Buffer
TIM-3 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-495P - TIM-3 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1315 - Human Tonsil Tissue Lysate
Species Reactivity
H, D
Protein Accession Number
NP_116171.3
Short Description
TIM3 / HAVCR2
This product belongs to the following categories:
Related Products
References- Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases. Trends Mol Med. 2005 Aug;11(8):362-9. Review.
Datasheet 08-01W
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