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Primary Antibodies
TERT Antibody
Background
Telomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG)n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human TElomerase Reverse Transcriptase), a template RNA called hTR, and telomerase-associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed; TERT transcription is a primary mechanism for regulation of telomerase activity.
Additional Names
TERT, EC 2.7.7.49, EST2, HEST2, HTERT, TCS1, TP2, TRT, hest2, telomerase catalytic subunit, tert
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Description
Left: Immunohistochemistry staining of TERT in lung, bronchiole (formalin-fixed paraffin-embedded) using TERT antibody.
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Purification
Sterile Filtration
Clonality / Clone
This is a polyclonal antibody.
Host
TERT antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
TERT antibody was raised against a region near the carboxy terminal end of TERT. (Human)
Application
TERT antibody can be used for detection of TERT by WB, IP, IHC-parafin (2.5 μg/ml).
Tested Application
WB, IHC, IP
Buffer
TERT antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
TERT antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
Rb
Formulation
Weight: 50 μg
Volume: 50 μl
Concentration: 1 mg/ml
Short Description
Telomerase Catalytic Subunit
This product belongs to the following categories:
Datasheet 09-01W
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