|
Primary Antibodies
TEM1 Antibody
Background
Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.
Additional Names
TEM1 (CT), Tumor endothelial marker 1, endosialin, CD248, CD164L1
|
Description
Left: Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/ml.
|
Source
TEM1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of the human TEM1.
Clonality / Clone
This is a polyclonal antibody.
Host
TEM1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
TEM1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1320 - Human Colon Tissue Lysate
Species Reactivity
H,M,R
Protein Accession Number
NP_065137
Data Sheet Version
08-02W
Related Products
References
- Rettig WJ, Garin-Chesa P, Healey JH, et al. Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.
- Christian S, Ahorn H, Koehler A, et al. Molecular cloning and characterization of endosialin, a C-type lectin-like cell surface receptor of tumor endothelium. J. Biol. Chem. 2001; 276:7408-14.
- Nanda A and St Croix B. Tumor endothelial markers: new targets for cancer therapy. Curr. Opin. Oncol. 2004; 16:44-9.
- Nanda A, Karim B, Peng Z, et al. Tumor endothelial marker 1 (TEM1) functions in the growth and progression of abdominal tumors. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.
|
|
