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Primary Antibodies
TDP43 Antibody
Background
TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3’ splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences. At least two isoforms are known to exist for this protein; this TDP43 antibody will recognize both isoforms.
Additional Names
TDP43 (NT), TAR DNA binding protein, TARDBP
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Description
Left: Western blot analysis of TDP43 in HeLa cell lysate with TDP43 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/ml.
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Other Product Images
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Source
TDP43 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TDP43.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TDP43 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4283P - TDP43 Peptide
Storage
TDP43 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
130750552
Protein Accession Number
ABO32290
Short Description
(NT) TAR DNA binding protein
This product belongs to the following categories:
Related Products
References- Ou SH, Wu F, Garcia-Martinez LF, et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J. Virol.1995; 69:3584-96.
- Buratti E, Dork T, Zuccato E, et al. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping. EMBO J. 2001; 20:1774-84.
- Neumann M, Sampathu DM, Kwong LK, et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 2006; 314:42-3.
Datasheet 08-03W
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