|
Primary Antibodies
TANK Antibody
Background
TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κB. This activation of NF-κB occurs through an association with the kinases IKKε and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IκB complex. This suggests that in addition to the possibility that TBK1 and IKKε activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κB proteins. At least two isoforms of TANK are known to exist.
Additional Names
TANK (CT), TRAF family member-associated NF-kappaB activator, I-TRAF
|
Description
Left: Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of TANK in rat spleen tissue with TANK antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
TANK antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human TANK.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TANK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TANK antibody can be used for the detection of TANK by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3877P - TANK Peptide
Storage
TANK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
- Cat. No. 1466 - Rat Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
19743569
Protein Accession Number
NP_004171
Short Description
(CT) TRAF, TBK1, innate immunity
This product belongs to the following categories:
Related Products
References- Cheng G and Baltimore D. TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-κB activation. Genes Dev. 1996; 10:963-73.
- Rothe M, Xiong J, Shu HB, et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Natl. Acad. Sci. USA 1996; 93:8241-6.
- Pomerantz JL and Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J. 1999; 18:6694-704.
- Chariot A, Leonardi A, Muller J, et al. Association of the adaptor TANK with the IκB kinase (IKK) regulator NEMO connects IKK complexes with the IKKε and TBK1 kinases. J. Biol. Chem. 2002; 277:37029-36.
Datasheet 08-02W
|
|
