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Primary Antibodies
TAK1 Antibody
Background
TAK1 (TGF-&betta; activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7) (1). It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-Β and members of the Toll-IL-1R (TIR) superfamily (2), thus acting as an intermediate in both proliferative and innate and adaptive immune responses (3). TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3 (4,5). Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation (6), while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity (5).
Additional Names
TAK1 (NT), MAP3K7
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Description
Left: Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
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Source
TAK1 antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino-terminus of human TAK1 isoform a.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TAK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TAK1 / MAP3K7 (N-Terminus) Peptide (Cat. No. 3385P)
Application
TAK1 antibody can be used for the detection of TAK1 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3385P - TAK1 Peptide
Storage
TAK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1471 - Rat Thymus Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
2924624
Protein Accession Number
BAA25025
Short Description
(NT) TGF-β activated protein kinase
This product belongs to the following categories:
Related Products
References- Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11.
- Irie T, Muta T, and Takeshige K. TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-κB in lipopolysaccharide-stimulated macrophages. FEBS Lett. 2000; 467:160-4.
- Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
- Jiang Z, Ninomiya-Tsuji J, Qian Y, et al. Interleukin-1 (IL-1) receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. Mol. Cell Biol. 2002; 22:7158-67.
Datasheet 08-02W
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