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Primary Antibodies
TAF(I)48 Antibody
Background
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
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Description
Left: Western blot analysis of TAF (I) 48 expression in Jurkat (A), HepG2 (B) and 293T (C) cell lysates using anti-TAF (I) 48.
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Purification
Rabbit IgG is purified by Protein A/G affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
TAF(I)48 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
TAF(I)48 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human TAF1A.
Application
TAF(I)48 antibody can be used for Western blot at a suggested dilution of 1.0 μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
TAF(I)48 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1211 - HepG2 Cell Lysate
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_005672
Molecular Weight
53 kDa
Short Description
Anti-TAF(I)48
This product belongs to the following categories:
Related Products
References- Di Pietro, C. et al (2000) Cytogenet. Cell Genet. 89 (1-2) 133-136.
Datasheet 0802-W
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