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Primary Antibodies
TAF1A Antibody
Background
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. TAF1A encodes the smallest SL1-specific TAF.
Additional Names
TAF1A
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Description
Left: Western blot analysis of TAF1A in Jurkat Cell Lysate using TAF1A antibody at 0.5 μg/ml (10% gel).
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
TAF1A antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1A.
Application
TAF1A antibody can be used for detection of TAF1A by ELISA at 1:1562500. TAF1A antibody can be used for detection of TAF1A by western blot at 0.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB, IHC
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store TAF1A antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_647603
Molecular Weight
40kDa
Short Description
TAF1A (TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa)
This product belongs to the following categories:
References- Di et al., (2000) Cytogenet. Cell Genet. 89 (1-2), 133-136.
Datasheet 10-01W
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