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Primary Antibodies
TACE Antibody
Background
Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
Additional Names
TACE (CT), adam17
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Description
Left: Western blot analysis of TACE in HeLa and Jurkat whole cell lysate in the absence or presence of blocking peptide (Cat. No. 1131P) with TACE antibody at 1:500 dilution.
Below: Immunocytochemistry of TACE in HeLa cells with TACE antibody at 10 μg/ml.
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Other Product Images
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Source
TACE antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human TACE This sequence differs from those of mouse and rat TACE by one amino acid.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
TACE antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human TACE (C-Terminus) Peptide (Cat. No. 1131P)
Application
TACE antibody can be used for detection of TACE by Western blot at 1:500 to 1:2000 dilution. 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1131P - TACE Peptide
Storage
TACE antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1205 - Jurkat Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
73747889
Protein Accession Number
NP_003174
Short Description
(CT) TNFα converting enzyme
This product belongs to the following categories:
Related Products
References- Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 1997;385:729-733
- Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-α. Nature 1997;385:733-736
- Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-α converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74
- Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4
Datasheet 08-02W
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