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Primary Antibodies
Sumo Antibody
Background
The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.
Additional Names
Sumo, small-ubiquitin-like modifier 1, ubiquitin-like protein 1, GAP-modifying protein 1
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Description
Left: Western blot analysis of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 μg/ml.
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Other Product Images
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Source
Sumo antibody was raised against a 14 amino acid peptide from near the amino terminus of human sumo .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Sumo antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Sumo antibody can be used for detection of sumo by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3969P - Sumo Peptide
Storage
Sumo antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1209 - HL-60Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
42490984
Protein Accession Number
AAH66306
Short Description
small ubiquitin-like modifier 1
This product belongs to the following categories:
Related Products
References- Dohmen RJ. Sumo protein modification. Biochim. Biophys. Acta 2004; 1695:113-31.
- Matunis MJ, Coutavas E, and Blobel G. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 1996; 135:1457-70.
- Gill G. Something about SUMO inhibits transcription. Curr. Opin. Genet. Dev. 2005; 15:536-41.
- Desterro JM, Rodriguez MS, and Hay RT. SUMO-1 modification of IkappaBalpha inhibits NFkappaB activation. Mol. Cell 1998; 2:233-9.
Datasheet 08-02W
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