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Primary Antibodies
Smac Antibody
Background
The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
Additional Names
Smac (CT), DIABLO
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Description
Left: Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 μg /ml.
Below: Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 ug/ml.
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Other Product Images
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Source
Smac antibody was raised against a peptide corresponding to amino acids 225 to 239 of human Smac/DIABLO .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Smac antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Smac / DIABLO (C-Terminus) Peptide (Cat. No. 2409P)
Application
Smac antibody can be used for detection of Smac/DIABLO by Western blot at 0.5 to 1 μg/ml. Human heart tissue lysate can be used as positive control and a 25 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2409P - Smac Peptide
Storage
Smac antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
- Cat. No. 1316 - Human Ovary Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
9454219
Protein Accession Number
AAF87716
Short Description
(CT) IAPs binding protein
This product belongs to the following categories:
Related Products
References- Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. M Cell. 2000;102(1):33-42.
- Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000;102(1):43-53.
- Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES. Molecular Determinants of the Caspase-promoting Activity of Smac/DIABLO and Its Role in the Death Receptor Pathway. J Biol Chem. 2000;275(46):36152-36157.
- Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature. 2000;406(6798):855-62.
Datasheet 08-02W
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