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Primary Antibodies
Slug Antibody
Background
Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-β. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family. Slug antibody has no cross-reactivity to Snail protein.
Additional Names
Slug (IN1), Neural crest transcription factor slug, Snail homolog 2
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Description
Left: Western blot analysis of Slug in human kidney cell lysate with Slug antibody at in (A) 1 and (B) 2 μg/ml.
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Source
Slug antibody was raised against a 14 amino acid peptide from near the center of human Slug .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Slug antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Slug antibody can be used for detection of Slug by Western blot at 1 to 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3957P - Slug Peptide
Storage
Slug antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M
Protein GI Number
11134406
Protein Accession Number
O43623
Short Description
(IN1) a member of the Snail family of C2H2-type zinc finger transcription factors
This product belongs to the following categories:
Related Products
References- Neito MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 1994; 264:835-9.
- Martinez-Estrada OM, Culleres A, Soriano FX, et al. The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells. Biochim J. 2006; 394:449-57.
- Shioiri M, Shida T, Koda K, et al. Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients. Br. J. Cancer 2006; 94:1816-22.
- Wu WS, Heinrichs S, Xu D, et al. Slug antagonizes p53-mediated apoptosis of hematopoietic progenitors by repressing puma. Cell 2005; 123:641-53.
Datasheet 08-02W
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