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Primary Antibodies
Ski Antibody
Background
TGF-β is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.
Additional Names
Ski, c-ski, v-ski sarcoma viral oncogene homolog
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Description
Left: Western blot analysis of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/ml.
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Other Product Images
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Source
Ski antibody was raised against a 14 amino acid peptide from near the amino terminus of human Ski.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Ski antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
SkiP antibody can be used for detection of Ski by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IF
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 2259P - Ski Peptide
Storage
Ski antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H
Protein GI Number
134517
Protein Accession Number
P12755
Short Description
A homolog of a retroviral oncogene
This product belongs to the following categories:
Related Products
References- Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
- Li Y, Turck CM, Teumer JK, et al. Unique sequence, ski, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
- Luo K. Ski and SnoN: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
- Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.
Datasheet 08-02W
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