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Primary Antibodies
STIM1 Antibody
Background
In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
Additional Names
STIM1, Stromal interaction molecule 1
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Description
Left: Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
STIM1 antibody was raised against a 24 amino acid peptide from near the carboxy terminus of human STIM1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
STIM1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
STIM1 antibody can be used for detection of STIM1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4119P - STIM1 Peptide
Storage
STIM1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1409 - Mouse Thymus Tissue Lysate
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
21070997
Protein Accession Number
NP_003147
Short Description
a calcium sensor that activates the CRAC channels
This product belongs to the following categories:
Related Products
References- Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5.
- Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.
Datasheet 08-02W
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