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Catalog Number:

46-444


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

STELLAR Antibody

Background

STELLAR Antibody.

Additional Names

STELLAR, germ and embryonic stem cell enriched protein STELLA, developmental pluripotency associated 3 pseudogene, stella-like, STELLA;

Source

STELLAR antibody was raised against a synthetic peptide of STELLAR.

Purification

STELLAR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

STELLAR antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 26kDa band in human ovary and placenta lysates after 0.1μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.9kDa according to NP_954980.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.

Tested Application

E

Buffer

STELLAR antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H

Protein Accession Number

NP_954980.1

Short Description

STELLAR

This product belongs to the following categories:

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References

  1. Ezeh UI, Turek PJ, Reijo RA, Clark AT. Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma. Cancer. 2005 Nov 15;104(10):2255-65.


Datasheet 08-01W

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