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Primary Antibodies
STAT3 Antibody
Background
The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and proliferation), depending upon the cell type. STATs are activated by ligand binding to cytokine receptors and other receptors associating with Janus kinase (JAK) family members that phosphorylate STAT proteins, causing them to assemble and translocate to the nucleus. In the nucleus, phosphorylated STAT protein complexes bind to DNA and regulate the transcription of particular genes. The epitope recognized by this antibody is located in the N-terminal region of STAT3. An antibody that reacts with the C-terminal region of STAT3 is available.
Source
STAT3 antibody was produced against recombinant protein, corresponding to the N-terminus (amino acid residues 1-170) of human STAT3, expressed in E. coli.
Clonality / Clone
This is a monoclonal antibody.
Host
STAT3 antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Specificity
Human, mouse and rat.
Application
STAT3 antibody is suitable for use in Western blotting.
Tested Application
WB
Buffer
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Storage
Store at 2-8˚C. For long term storage, aliquot into small volumes and store at –20˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Positive Control
- Human MCF-7 cells and mouse L929 cells.
Species Reactivity
H, M, R
Formulation
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Short Description
STAT3
This product belongs to the following categories:
References- Booz, G.W., et al. (2003) Angiotensin II effects on STAT3 phosphorylation in cardiomyocytes: evidence for Erk-dependent Tyr705 dephosphorylation. Basic Res. Cardiol. 98:33-38.
- Boeuf, H., et al. (2001) The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells. J. Biol. Chem. 276:46204-46211.
- Stephens, J.M., et al. (1998) Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes. J. Biol. Chem. 273:31408-31416.
- Wen, Z. (1995) Maximal activation of transcription by STAT1 and STAT3 requires both tyrosine and serine phosphorylation. Cell 82:241-250.
Datasheet 0801-W
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