|
Primary Antibodies
STAT3 Antibody
Background
The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and proliferation), depending upon the cell type. STATs are activated by ligand binding to cytokine receptors and other receptors associating with Janus kinase (JAK) family members that phosphorylate STAT proteins, causing them to assemble and translocate to the nucleus. In the nucleus, phosphorylated STAT protein complexes bind to DNA and regulate the transcription of particular genes. The epitope recognized by this antibody is located in the N-terminal region of STAT3. An antibody that reacts with the C-terminal region of STAT3 is available.
Source
STAT3 antibody was produced against recombinant protein, corresponding to the N-terminus (amino acid residues 1-170) of human STAT3, expressed in E. coli.
Clonality / Clone
This is a polyclonal antibody.
Host
STAT3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Specificity
Human, mouse and rat. Other species were not tested.
Application
STAT3 antibody has been used for Western blotting applications.
Tested Application
WB
Buffer
Purified immunoglobulin in phosphate buffered saline containing 1% BSA.
Storage
Store at 2-8˚C for up to one month. For long term storage, apportion into working aliquots and store at -20˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Positive Control
- Human A431, mouse 3T3L1 cells.
Species Reactivity
H, M, R
Formulation
Purified immunoglobulin in phosphate buffered saline containing 1% BSA.
Short Description
STAT3
This product belongs to the following categories:
References- Booz, G.W., et al. (2003) Angiotensin II effects on STAT3 phosphorylation in cardiomyocytes: evidence for Erk-dependent Tyr705 dephosphorylation. Basic Res. Cardiol. 98:33-38.
- Boeuf, H., et al. (2001) The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells. J. Biol. Chem. 276:46204-46211.
- Dumler, I., et al. (1999) Urokinase induces activation and formation of Stat4 and Stat1-Stat2 complexes in human vascular smooth muscle cells. J. Biol. Chem. 274:24059-24065.
- Stephens, J.M., et al. (1998) Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes. J. Biol. Chem. 273:31408-31416.
Datasheet 0801-W
|
|
